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Figure 6 | BMC Biotechnology

Figure 6

From: Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitor

Figure 6

Lineweaver-Burk Plot of inhibition of PLAP activity by purified GLE4.The enzyme activity of PLAP is inhibited in a competitive manner. Km of the enzyme: 5.5 mM; Km of the enzyme with GLE4: 40 mM. Inset shows % inhibition of enzyme activity by varying amount of purified scFv GLE4 (This clone also shows a higher binding [by ELISA] to PLAP as compared to BAP or IAP).

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BMC Biotechnology

ISSN: 1472-6750

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