Skip to main content
Figure 4 | BMC Biotechnology

Figure 4

From: Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitor

Figure 4

Binding features of Type I, Type II and Type III antibodies on MBPhE. 4A.Representative binding of Type I antibodies on MBPhE. The binding of these antibodies was inhibited by L-Phe-Gly-Gly + pNPP but not by L-Gly-Gly-Gly + pNPP. The unconjugated magnetic beads served as a negative control. The black bars on top indicate SD. 4B.Representative binding of Type II antibodies on MBPhE. The binding of these antibodies was inhibited both by L-Phe-Gly-Gly + pNPP and L-Gly-Gly-Gly + pNPP and by substrate alone. The unconjugated magnetic beads served as a negative control. The black bars on top indicate SD. 4C.Representative binding of Type III antibodies on MBPhE. The binding of these antibodies was not inhibited by L-Phe-Gly-Gly + pNPP or L-Gly-Gly-Gly + pNPP. The unconjugated magnetic beads served as a negative control. The black bars on top indicate SD.

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us