Protein | Affinity chromatography ligand | Elution conditions | Expression system | Production yield (mg/l)a |
---|---|---|---|---|
AVR2 | D-biotin | 0.5 M acetic acid | BEVSb | 0.8 |
AVR2-b | D-biotin | 0.5 M acetic acid | E. coli c | 4.9 |
AVR2(I109K) | D-biotin | 0.5 M acetic acid | BEVS | 9.0 |
AVR4d | 2-iminobiotin | 50 mM Na-Ac + 100 mM NaCl | BEVS | 6.1 |
AVR4-bd | 2-iminobiotin | 50 mM Na-Ac + 100 mM NaCl | E. coli | 21.9 |
AVR4(K109I)d | 2-iminobiotin | 50 mM Na-Ac + 100 mM NaCl | BEVS | 16.2 |
AVR4(K109I)-bd | 2-iminobiotin | 50 mM Na-Ac + 100 mM NaCl | E. coli | 6.7 |
AVR6-be | D-biotin | 2 M acetic acid | E. coli | 8.3 |
AVD(K111I) | 2-iminobiotin | 50 mM Na-Ac + 100 mM NaCl | BEVS | 11.5 |
AVD(K111I)-b | 2-iminobiotin | 50 mM Na-Ac + 100 mM NaCl | E. coli | 0.7 |