Figure 4

Biotin dissociation analysis. (A) Temperature-dependence of biotin dissociation rates measured by the [³H]biotin dissociation assay. Radiobiotin dissociation rate constants measured for the proteins are plotted as a function of temperature. The models for dissociation rate constants obtained by global fit analysis [41] are shown by lines and the determined individual dissociation rates by symbols. The dissociation rate constants determined previously for avidin and AVR4-b are also shown [39]. (B) Relative dissociation rate constants of the AVRs and avidin mutant K111I compared to that of avidin. The individual dissociation rates as well as global fit analysis models of the dissociation rate are divided by the dissociation rate constants of avidin obtained from global fit analysis. The data are plotted using a logarithmic y-axis.