Table 5 Yield and STR genotypes from NTC samples
From: Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance
gDNA Input (ng) | Yield | |||
|---|---|---|---|---|
N | Mean RT-PCR (ng) | Median ssDNA (%) | Median total DNA (ng) | |
gDNA | - | - | - | - |
1 | 4 | 135 | 51.5 | 12230 |
10 | 4 | 0 | 50.8 | 11771 |
25 | 4 | 0 | 55 | 12340 |
50 | 4 | 24 | 45 | 12395 |
100 | 4 | 220 | 48.6 | 12686 |
200 | 4 | 0 | 54 | 14879 |
STR | ||||
gDNA Input (ng) | N | False Positive Rate (%) 1 | Mean Height Allele 1 | Mean Height Allele 2 |
gDNA | 64 | 6 | 93 | 93 |
1 | 128 | 5 | 2185 | 2199 |
10 | 128 | 0 | - | - |
25 | 128 | 0 | - | - |
50 | 128 | 92 | 375 | 393 |
100 | 128 | 92 | 571 | 571 |
200 | 128 | 2 | 2360 | 2360 |