Figure 2
Xist/Sry real-time PCR efficiency in the presence or absence of diluted denaturant. Standard curve used for the quantification of multiplexed Xist and Sry amplicons, obtained by serial dilution of male mouse genomic DNA at known copy number (abscissa). The Xist (light purple) and Sry (green) signals had very similar CT values (ordinate) at every DNA concentration tested, because the two PCR reactions had been optimized to be equally efficient. The addition of denaturing solution at the concentration used in the PurAmp assays did not inhibit PCR (red, Xist; blue, Sry), as also seen in the case of the hsp70i amplicon.