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Figure 4 | BMC Biotechnology

Figure 4

From: CpG-island fragments from the HNRPA2B1/CBX3genomic locus reduce silencing and enhance transgene expression from the hCMV promoter/enhancer in mammalian cells

Figure 4

The 8.0 kb RNP CpG island fragment gives a high proportion of clonal CHO-K1 cell lines showing high-level, stable expression of EGFP. Following stable transfection of CHO-K1 cells clonal lines were derived by limiting dilution. Median fluorescence levels were measured for each clone by FACScan analysis after 22 generations of culturing with drug selection. A. Histograms representing the median fluorescence levels for clones generated with hCMV-EGFP (n = 112) and 8.0 kb RNP-EGFP (n = 86). B. FACscan analysis of two clonal lines described in A. Clonal cell lines were subcultured continuously with or without drug selection for a further 213 generations, with analysis after 38 and 213 generations. Figures for gate M1 are % cells showing greater than 1000 fluorescence units.

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BMC Biotechnology

ISSN: 1472-6750

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