Figure 9
Elution of retained labeled liposomes with Rhod-PE and calcein by passing non-labeled liposomes. Liposomes, labeled with rhod-PE (fluorescence represented with red triangles, 
) and containing calcein, 500 μM (fluorescence represented with blue circles, 
) were chromatographied on G25M. Fractions of 1.5 mL were collected. The first arrow indicates the injection of unlabeled liposomes on the column and the second arrow indicates the injection of 0.5% TX-100 on the top of the columns.