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Figure 6 | BMC Biotechnology

Figure 6

From: Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells

Figure 6

Effect of BS upon the human keratinocytes transduced with LBmSN vector On day 1, the irradiated PA317/LBmSN cells (30 Gy) and the human keratinocytes were seeded together at 3.3 × 105 and 5 × 104 cells per well respectively in a 12 well plate. Two days later, the medium was replaced with a fresh one containing the indicated concentrations of antibiotics. The cells were stained with Rhodamine B after 8 days. (A) no antibiotic; (B) 800 μg/ml G418; (C) 8 μg/ml BS; (D) 12 μg/ml BS; (E) 16 μg/ml BS; (F) 32 μg/ml BS

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