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Figure 2 | BMC Biotechnology

Figure 2

From: A faster way to make GFP-based biosensors: Two new transposons for creating multicolored libraries of fluorescent fusion proteins

Figure 2

In-frame <EYOR> transposition events in the voltage sensitive integral membrane motor protein, Prestin. Visual screening of transiently transfected HEK-293 cells revealed truncated Prestin-YFP fusion proteins generated by in-frame <EYOR> transpositions (A) (scale bar = 20 μm). Alternate digestion and re-ligation of an in-frame clone with either Srf I or Asc I produces identical full-length YFP- (B) and CFP-fusion proteins (C). In-frame <EYOR> insertions in Prestin are identified by the first of 3 target amino acids duplicated during transposition (D). Redundant insertions are indicated by the number of recovered clones (e.g. 3x).

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BMC Biotechnology

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