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Table 2 PPV detection in non-inoculated apical leaves one week and six weeks after inoculation. DAS-ELISA absorbance values from ihprolC-PP197 transgenic and wild type plants are reported.

From: Expression of self-complementary hairpin RNA under the control of the rolCpromoter confers systemic disease resistance to plum pox virus without preventing local infection

 

Lines

 

8

 

13

 

29

 

2

 

9

 

Plant

1 week

6 weeks

1 week

6 weeks

1 week

6 weeks

1 week

6 weeks

1 week

6 weeks

1

0.160

0.360*

0.061

0.002

0.012

0.002

0.056

0.001

0.097

0.023

2

0.129

0.080

0020

0.012

0.012

0.298*

0.044

0.010

0.089

0.027

3

0.119

0.069

0.054

0.041

0.005

0.086

0.112

0.038

0.079

0.031

4

0.126

0.066

0.020

0.022

0.070

0.008

0.092

0.075

0.047

0.018

5

0.094

0.355*

0.060

0.083

0.003

0.002

0.137

0.004

0.052

0.014

6

0.081

0.067

0.062

0.009

0.011

0.005

  

0.117

0.001

7

0.057

0.072

  

0.006

0.424*

  

0.131

0.086

8

0.049

0.036

  

0.008

0.873*

  

0.104

0.047

9

0.080

0.032

      

0.387

0.021

10

0.084

0.082

        

11

0.075

0.640*

        

WT

0.720

 

1.520

 

0.650

 

0.450

 

0.450

 
  1. Samples with absorbance values higher than 0.097 were considered virus-infected. This threshold was calculated as mean absorbance value of the mock inoculated plants plus 3 times the standard deviation of the mean.* Transgenic plants showing leaf mottling (i.e. mild symptoms of PPV infection).