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Table 1 Chimeric primers for RT and primers for PCR.

From: A new reverse transcription-polymerase chain reaction method for accurate quantification

Primers

Sequences

For RT

 

Rat VHL

 

Short

5'-CGGCTCGTGACTGCTATCTTC AATGTGATGTTGGCAAAAAT-3'

Long

5'-CGGCTCGTGACTGCTATCTTC(N)nAATGTGATGTTGGCAAAAAT-3'

Rat Collagen I

 

Short

5'-CGGCTCGTGACTGCTATCTTC CCTTGGCACCATCGTTGC-3'

Long

5'-CGGCTCGTGACTGCTATCTTC(N)nCCTTGGCACCATCGTTGC-3'

For PCR

 

Rat VHL

 

Upper

5'-GGGACCCATGATGGACTTCT-3'

Lower

5'-CGGCTCGTGACTGCTATCTTC-3'

Rat Collagen I

 

Upper

5'-CCCTGGTGAACGTGGTGTAC-3'

Lower

The same as the VHL lower primer

  1. Gene-specific sequences: letters in italic; a universal sequence: bold letters; the linkers (N)n: either (A)10, (C)10, (G)10, (AT)5, or (A)5(G)5(A)5.
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BMC Biotechnology

ISSN: 1472-6750

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