Table 4 Partitioning of cell-linked and extracellular COX after phase-separation of Triton X-114
COX total units | COX activity (U/ml) | K | ||||
|---|---|---|---|---|---|---|
% Triton X-114 | % volume of rich phase | Rich phase | Depleted phase | Rich phase | Depleted phase | |
Cell-linked | ||||||
1 | 10.8 | 8.5 | 234.8 | 78.7 | 263.2 | 0.30 |
2 | 15.9 | 137.9 | 136.5 | 867.3 | 162.3 | 5.34 |
3 | 29.6 | 276.5 | 87.1 | 934.1 | 123.7 | 7.55 |
4 | 37.5 | 278.0 | 86.5 | 741.3 | 138.4 | 5.35 |
6 | 41.8 | 280.0 | 85.0 | 669.8 | 146.0 | 4.58 |
Extracellular | ||||||
1 | 6.4 | 5.0 | 113.7 | 78.2 | 121.5 | 0.64 |
2 | 9.3 | 22.8 | 99.7 | 245.7 | 109.7 | 2.24 |
3 | 10.7 | 58.8 | 69.4 | 550.5 | 77.7 | 7.08 |
4 | 12.8 | 70.5 | 55.1 | 552.3 | 63.1 | 8.75 |
6a | 15.3 | 84.0 | 41 | 547.9 | 48.4 | 11.32 |
6 | 15.3 | 390 | 41 | 2544 | 48.4 | 52.60 |