Table 2 MMV applications developed to date

Multiple (1024) conditions generation (Multiple-conditioner)

Generation of 1024 different conditions in MMV for lysozyme crystallization.

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Applicable to iPS primary induction factor screening

Semi-infinite replica Formation (D2D, C2C, and D2D2P)

Replication of DNA and cells in MMV perpetually. Besides, DNA processed to protein (D2D2P) replica.

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Proteins replica can be perpetually generated by two steps of D2D and D2P, i.e., D2D2P.

NGS-non-dependent microbiome analysis (NNMA)

Single-cell isolation, DNA extraction, single-cell random PCR of microbiome samples in MMV, and processing of PCR products by the Genome Profiling (GP) method.

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Serves as single cell isolation and analysis tool

Multistep function-based screening (POMM)

Operation of all steps involved in DNA amplification, in vitro transcription and translation; identification of functional peptides in MMV.

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Screening tool with samples addressed

All-In-One/All-At-Once assay (A^I/_AO)

Screening of apoptosis-inducing peptides against cancer cells.

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Applicable to monoclonal antibody screening