Figure 8
From: Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85B
Analytical SEC of antigen 85 B (batch 09-2/1). A) Sample stored at 2 – 8°C, B) sample boiled for 20 min at 100°C. 500 μL of sample were applied to Superdex 200 10/300 GL column. Running buffer was PBS pH 7.4. Standards: 1. 669 kDa 17 min, 2. 443 kDa 20 min, 3. 200 kDa 23 min, 4. 150 kDa 24 min, 5. 66 kDa 26 min, 6. 29 kDa 31 min, 7. 12.4 kDa 34 min, 8. 6.5 kDa 37 min.