Figure 5
From: Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85B
A-85 B sandwich antigen titration ELISA. 100 ng of capture scFv-Fc were directly coated to the wells. After blocking different dilutions of antigen were applied and incubated. Detection of bound antigen with: A) MFU50-A10-scFv-Fc-HRP at ~0.05 μg/mL and development with TMB. Negative control (BSA) A450 = 0.02, B) MFU50-C10-scFv-Fc-HRP at ~0.05 μg/mL and development with TMB. Negative control (BSA) A450 = 0.05, C) MFU50-D4-scFv-Fc-HRP at ~0.05 μg/mL and development with TMB. Negative control (BSA) A450 = 0.02.