Table 2 Combinations of GenSolve, Qiagen and NucleoSpin protocols for DNA extraction from DBS: GQ versus GN and Gn methods
From: Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling
65°C, 65 min | ||||||||
|---|---|---|---|---|---|---|---|---|
Sample code | DNA (ng/μl) | DNA (ng) | 260/280 | 260/230 | 260/280 | 260/230 | Protocol | |
NCS 31a | 5.77 | 242 | 1.70 | 0.42 | 1.55 | 0.41 | GQ | |
NCS 31b | 8.87 | 426 | 1.81 | 0.95 | 1.54 | 0.71 | GN | |
NCS 30a | 12.8 | 538 | 1.88 | 0.79 | 1.76 | 0.76 | GQ | |
NCS 30b | 24.4 | 1171 | 1.84 | 1.22 | 1.90 | 1.51 | GN | |
NCS 29a | 20.7 | 828 | 1.85 | 1.13 | 1.76 | 1.00 | GQ | |
NCS 29b | 23.1 | 1063 | 1.93 | 1.38 | 1.85 | 1.37 | GN | |
Precipitation buffer in GN changed to ethanol, leading to protocol Gn | NCS 22a | 9.85 | 394 | 1.81 | 0.50 | 1.79 | 0.48 | GQ |
NCS 22b | 8.00 | 368 | 1.90 | 0.83 | 1.79 | 0.75 | Gn | |
NCS 21a | 24.3 | 972 | 1.83 | 0.84 | 1.83 | 0.90 | GQ | |
NCS 21b | 27.7 | 1274 | 1.64 | 0.54 | 1.83 | 0.61 | Gn | |
NCS 20a | 15.0 | 600 | 1.78 | 0.56 | 1.77 | 0.60 | GQ | |
NCS 20b | 14.4 | 662 | 1.70 | 0.76 | 1.74 | 0.89 | Gn |