Table 1 Primers used in the amplification of shRNA pools from sorted cell
Primer sequence | Id | Info |
|---|---|---|
CCGAGGTGGGCAGTCAATCAATCT | DK633outerr | outer primer |
ACGCCATTTTGCAAGGCAT | DK637altouterf | outer primer |
454-adaptor primers | ||
Lowercase letters provide a multiplexing code to allow separate PCR reactions to be pooled and sequenced together | ||
GCCTCCCTCGCGCCATCAGagcTTTCTTGGGTAGTTTGCAGTTT | DK621ACodeAGC | ‘A’ type, nested |
GCCTCCCTCGCGCCATCAGtgaTTTCTTGGGTAGTTTGCAGTTT | DK621ACodeTGA | ‘A’ type, nested |
GCCTCCCTCGCGCCATCAGcagTTTCTTGGGTAGTTTGCAGTTT | DK621ACodeCAG | ‘A’ type, nested |
Type A primers are identical except for the 3 nt’s in small letters | ||
GCCTTGCCAGCCCGCTCAGtactgacTTGTACAAGAAAGCTGGGTAAG | DK622BCodeTActgaC | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGtacgatcTTGTACAAGAAAGCTGGGTAAG | DK622BCodeTAcgatC | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGtacagtcTTGTACAAGAAAGCTGGGTAAG | DK622BCodeTAcagtC | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGtagactcTTGTACAAGAAAGCTGGGTAAG | DK622BCodeTAgactC | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGtagcatcTTGTACAAGAAAGCTGGGTAAG | DK622BCodeTAgcatC | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGtacgtacTTGTACAAGAAAGCTGGGTAAG | DK622BCodeTAcgtaC | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGtagctacTTGTACAAGAAAGCTGGGTAAG | DK622BCodeTAgctaC | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGtagtcacTTGTACAAGAAAGCTGGGTAAG | DK622BCodeTAgtcaC | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGcactgagTTGTACAAGAAAGCTGGGTAAG | DK622BCodeCActgaG | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGcacgatgTTGTACAAGAAAGCTGGGTAAG | DK622BCodeCAcgatG | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGcacagtgTTGTACAAGAAAGCTGGGTAAG | DK622BCodeCAcagtG | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGcagactgTTGTACAAGAAAGCTGGGTAAG | DK622BCodeCAgactG | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGcagcatgTTGTACAAGAAAGCTGGGTAAG | DK622BCodeCAgcatG | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGcacgtagTTGTACAAGAAAGCTGGGTAAG | DK622BCodeCAcgtaG | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGcagatcgTTGTACAAGAAAGCTGGGTAAG | DK622BCodeCAgatcG | ‘B’ type, nested |
GCCTTGCCAGCCCGCTCAGcagtcagTTGTACAAGAAAGCTGGGTAAG | DK622BCodeCAgtcaG | ‘B’ type, nested |
Type B primers are identical except the 7 nt’s in small letters. | ||