Table 4 Bacterial strains and plasmids used in this study
Strains/plasmids | Genotype or description | Source |
|---|---|---|
Strains | Â | Â |
 E. coli DH5α | F-,supE44,ΔlacU169(φ80lacZΔM15),hsdR17, recA1,endA1, gyrA96, thi-1, relA1 | Life Technologies |
 E. coli BL21(DE3) | F-, ompT, hsdSB(rB-mB-), gal, dcm(DE3) | Life Technologies |
Plasmids | ||
 pG-KJE8 | Encoding DnaK-DnaJ-GrpE / GroES-GroEL | Takara |
 pGro7 | Encoding GroES-GroEL | Takara |
 pKJE7 | Encoding DnaK-DnaJ-GrpE | Takara |
 pTf16 | Encoding Tig | Takara |
 pColdTF | Expression vector | Takara |
 pET-30a (+) | Expression vector | Novagen |
 pGEX-6P-1 | Expression vector | GE Healthcare |
 pBAD/gIIIA | Expression vector | Invitrogen |