Figure 3From: Cloning, expression and biochemical characterization of the cholesterol oxidase CgChoA from Chryseobacterium gleumUV–vis absorbance spectrum of purified CgChoA. The typical absorption spectra for FAD was obtained (peaks at 370 nm and 470 nm; dashed line: 10 fold magnification) [17]. Inset: SDS-PAGE before and after purification of CgChoA. Lane 1: cell free extract; lane 2: protein after IMAC purification; lane 3: purified sample after SEC. The band corresponding to CgChoA is indicated by the arrow.Back to article page