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Figure 3 | BMC Biotechnology

Figure 3

From: Cloning, expression and biochemical characterization of the cholesterol oxidase CgChoA from Chryseobacterium gleum

Figure 3

UV–vis absorbance spectrum of purified CgChoA. The typical absorption spectra for FAD was obtained (peaks at 370 nm and 470 nm; dashed line: 10 fold magnification) [17]. Inset: SDS-PAGE before and after purification of CgChoA. Lane 1: cell free extract; lane 2: protein after IMAC purification; lane 3: purified sample after SEC. The band corresponding to CgChoA is indicated by the arrow.

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