Identifying inhibitory compounds in lignocellulosic biomass hydrolysates using an exometabolomics approach

BMC Biotechnology

Table 8 Compounds that caused synergistic effect with furfural or HMF at 0.5 g/l, tested using Bioscreen C Analyzer

Reference medium		LP	GR	OD
(MM with 20 g/l glucose)		7 h	0.105	1.2
	Added compound	Added compound only			Added compound + furfural or HMF
		LP	GR	OD	LP	GR	OD
Furfural	HMF	---	---	---	19 h	< 80%	---
0.5 g/l	HMF acid	---	---	---	16.5 h	---	---
15 h	Salicylic acid	---	< 60%	< 80%	15 h	< 40%	< 60%
90%	Vanillin	11 h	< 80%	---	17 h	< 80%	---
100%	Syringaldehyde	---	---	---	16.5 h	< 80%	---
	Levulinic acid	---	---	---	9 h	< 80%	---
HMF	2-furoic acid	---	---	---	8.5 h	< 80%	---
0.5 g/l	Pantoyllacton	---	---	---	9 h	< 80%	---
7 h	Salicylic acid	---	< 60%	< 80%	8.5 h	< 40%	< 60%
100%	Vanillin	11 h	< 80%	---	11 h	< 60%	---
100%	Syringaldehyde	---	---	---	11 h	---	---

LP: lag-phase: time needed to reach 2% (ODmax-ODmin) (h), GR: growth rate: the slope of the linear part of the OD curve (OD/h), OD: final OD. Values with% are relative growth rate and final OD compared to that in reference medium. ‘---’: no effect compared to reference medium; the ‘bold effects’ were relatively significant.

ISSN: 1472-6750