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Table 2 Effects of different chemicals on the activity of the purified recombinant PelN

From: Cloning, expression and characterization of a pectate lyase from Paenibacillus sp. 0602 in recombinant Escherichia coli

Chemicals

Relative activity (%)*

Concentration (mM)

0.5

1.0

None

100 ± 1

100 ± 1

Ca2+

729 ± 38

437 ± 1

Mg2+

125 ± 11

99 ± 3

Mn2+

75 ± 3

57 ± 5

Cu2+

79 ± 3

104 ± 11

Zn2+

116 ± 8

106 ± 7

Fe2+

82 ± 7

57 ± 3

Ba2+

12 ± 3

3 ± 0

K+

119 ± 8

100 ± 4

EDTA

nd

nd

Triton X-100

102 ± 9

100 ± 8

Tween-20

98 ± 2

116 ± 11

  1. nd: no detectable activity.
  2. *The activity under the standard conditions without any chemicals was used as the reference (100%), and data represent means ± SD (n = 4) relative to the untreated reference samples;
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BMC Biotechnology

ISSN: 1472-6750

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