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Figure 2 | BMC Biotechnology

Figure 2

From: Differences in virulence of pneumolysin and autolysin mutants constructed by insertion duplication mutagenesis and in-frame deletion in Streptococcus pneumoniae

Figure 2

Verification of D39 and the IDM- ply and - lytA mutants. (A) A product could only be amplified from D39 using primer set ply-P1/ply-P2. (B) The IDM-ply mutant was confirmed by the lack of an amplification product using the ply-P1 and ply-P2 primer set, and was shown to contain an inserted pVA891 and truncated ply using primers pairs ply-P2/pVA891-F and ply-P1/pVA891-R, respectively. (C) A product was only amplified from D39 using primer pair lytA-P1/lytA-P2. (D) The IDM-lytA mutant was verified by no amplification product using primers lytA-P1 and lytA-P2 and shown to have an inserted pEVP3 and truncated lytA using primer sets lytA-P2/pEVP3-F and lytA-P1/pEVP3-R, respectively. The locations of primers and predicted molecular weight of the PCR products for D39, IDM-ply, and IDM-lytA are given in Figure 1 and Table 1. M, molecular DNA marker.

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BMC Biotechnology

ISSN: 1472-6750

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