Figure 3

Morphological shaping of Oli-neu and COS7 cells by micropatterned PLL (A, D) High concentration (1 mg/mL) FITC-conjugated PLL lines were printed on top of a low-concentration (1 pg/mL) lawn of unconjugated PLL. (B, C) Oli-neu cells adhered preferentially to the high concentration lines, (E, F) COS7 cells showed a preference for the low-concentration PLL regions. C is a high magnification image overlaying FITC-PLL (green) from A and Oli-neu cells stained with rhodamine-phalloidin (red) for actin from B. F is a high magnification overlay of FITC-PLL and phalloidin-stained COS7 cells. Scale bars are 60 μm.