Figure 3From: Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease VRobust PCR-amplification of insert DNA fragments using deoxyinosine-containing primers. Analytical agarose gel electrophoresis of PCR products produced by Taq polymerase using either plasmid DNA (A) or E. coli colonies (B) as template material. Relative to the calculated Tm, annealing temperatures used for PCR cycling are indicated for each lane.Back to article page