Figure 4From: Systematic screening of glycosylation- and trafficking-associated gene knockouts in Saccharomyces cerevisiaeidentifies mutants with improved heterologous exocellulase activity and host secretionExtracellular His-tagged PCX from different hosts. SDS-PAGE, left, 10 μg per lane, and western blot, right, 1 μg per lane of extracellular his-tagged PCX from different hosts. From left to right: lane 1, PCX from the BY4741 strain; lane 2, PCX from the mnn10 null mutant; lane 3, PCX from the vps21 null mutant; lane 4, PCX from the pmt5 null mutant; and lane 5, PCX from the alg1-1 mutant. The protein marker is the Fermentas PageRuler Pre-stained Protein Ladder.Back to article page