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Figure 5 | BMC Biotechnology

Figure 5

From: Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papayaL.)

Figure 5

Real-time PCR analysis of coat protein (CP) in a dilution series of GE in non-GE papaya DNA. A) Linear regression obtained with CP primer pair with total nucleic acid mixtures of ‘Rainbow’ and ‘Waimanalo’. B) GE ‘Rainbow’ DNA diluted in non-GE ‘Waimanalo’ DNA. C) GE ‘SunUp’ DNA diluted in non-GE ‘Waimanalo’ DNA. Lanes in B and C, 1: water, 2–5: 3.125%, 1.56%, 0.75% and 0.038% transgenic DNA respectively. M: 1 kb DNA ladder (Fisher Scientific).

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BMC Biotechnology

ISSN: 1472-6750

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