Table 1 Putative proteins in the supernatant of a U. maydis MB215 culture grown on xylan detected by MALDI-TOF analysis
From: Identification of an endo-1,4-beta-xylanase of Ustilago maydis
Putative enzyme activity | Gene(s) | ScoreA | Mw[kDa] | pI | Sequence coverage [%]A | Number of peptidesA | CAZy familyB | Remark |
|---|---|---|---|---|---|---|---|---|
endo-1,4-beta-xylanase | um06350.1 | 87/111 | 23.8 | 9.41 | 24.0/18.1 | 4/3 | GH11 | xylan degradation |
arabinoxylan arabinofurano-hydrolase | um04309.1 | 149/142 | 36.3 | 9.00 | 21.1/13.6 | 5/3 | GH62 | xylan degradation |
glycosidase | um02727.1 | 179/180 | 25.1 | 9.27 | 26.7/26.7 | 3/3 | GH25 | D-glucan degradation |
endo-1,3(4)-beta-glucanase | um02134.1 | 331/58 | 41.5 | 5.84 | 17.3/2.3 | 4/1 | GH16 | D-glucan degradation |
glycosyl hydrolase | um06247.1 | 65/85 | 54.0 | 5.98 | 4.47/4.4 | 2/2 | Â | (hemi-) /cellulose degradation |
chitin deacetylase | um02689.1 | -/100 | 52.4 | 6.63 | -/6.0 | -/2 | CE4 | polysaccharide degradation |
lipase B | um01422.1 | 118/130 | 35.1 | 8.81 | 8.3/8.3 | 2/2 | Â | lipid degradation |
aspartic protease | um00064.1 | 316/338 | 41.5 | 5.80 | 17.1/17.1 | 4/4 | Â | peptide degradation |
alpha-galactosidase | um04503.1 | 153/138 | 60.5 | 6.58 | 8.6/4.7 | 3/2 | GH27 | galactomannan degradation |
spherulin 4 | um06157.1 | 237/150 | 35.5 | 9.67 | 24.7/14.5 | 4/3 | Â | spherulin-like protein |
choline dehydrogenase | um03246.1 | 222/176 | 67.9 | 6.30 | 8.0/6.5 | 5/3 | Â | glycine, serine and threonine metabolism |
uncharacterized protein | um05604.1 | 88/101 | 27.0 | 4.65 | 6.0/6.0 | 3/3 | Â | unknown |
uncharacterized protein | um01894.1 | 49/48 | 30.4 | 5.53 | 6.5/6.5 | 2/2 | Â | unknown |
uncharacterized protein | um00961.1 | 367/371 | 31.6 | 6.19 | 24.8/20.7 | 4/3 | Â | unknown |