Figure 1

T20 analyzer development. (A) Antibody sequence curation. Variable region protein sequences had Kabat numbering applied and the CDR regions identified. Variable heavy chain (VH), variable light kappa chain (VK), and variable light lambda chain (VL) sequences were curated into databases as full-length sequences or framework-only sequences (where the CDR regions were removed). The number of unique antibody sequences in each database is shown. (B) Defining the T20 score. Protein BLAST is used to determine the sequence identity of an input sequence with each database sequence. The percent identities of the Top 20 matched sequences are averaged to obtain the T20 score for a given input sequence. (C) Left: Details of obtaining the T20 Cutoff Human Databases. Right: Histogram of the T20 scores of a sample set of antibody sequences, indicating the T20 score cutoff and the 15% of sequences below this cutoff that were removed to form the T20 Cutoff Human Database for each chain type. The number of unique antibody sequences in each database is shown.