From: One single method to produce native and Tat-fused recombinant human α-synuclein in Escherichia coli
Purification step | Total protein (mg) | AS (mg) | AS purity a(%) | Yield b(%) |
---|---|---|---|---|
Whole cell extract c | 210 [185] d | 35 [22.2] | 16.7 [12.0] | 100 [100] |
Osmotic shock | 40 [29] d | 30 [20] | 75 [69] | 85 [90.1] |
HiTrap chromatography | 16 [11.8] e | 16 [11.8] | > 95 [> 95] | 53 [59] |