Figure 2From: One single method to produce native and Tat-fused recombinant human α-synuclein in Escherichia coliSpectral properties of recombinant native AS. In 20 mM potassium phosphate buffer, pH 7.4, 150 mM sodium chloride, after eliminating endotoxins. A) UV–vis Absorbance spectrum of AS, 3.2 mg/mL, 15°C. B) Protein fluorescence of AS; 0.1 mg/mL, excitation at 280 nm. C) Far-UV circular dichroism spectrum of AS; 0.1 mg/mL.Back to article page