Table 4 Kinetic parameters of BglMKg
Substrate | Temperature (°C) | K m (mM) | k cat (s-1) | k cat /K m (s-1mM-1) |
|---|---|---|---|---|
10 | 4.82 ± 1.10 | 3.99 ± 0.53 | 0.83 | |
ONPGal | 20 | 9.04 ± 1.39 | 12.43 ± 1.35 | 1.37 |
30 | 5.72 ± 0.60 | 14.73 ± 0.95 | 2.57 | |
10 | 0.13 ± 0.06 | 4.46 ± 0.28 | 34.31 | |
PNPGlc | 20 | 0.16 ± 0.03 | 11.74 ± 0.26 | 73.37 |
30 | 0.37 ± 0.04 | 22.45 ± 0.64 | 60.67 | |
10 | 73.89 ± 46.97 | 7.95 ± 3.99 | 0.11 | |
Lactose | 20 | 41.56 ±13.31 | 8.55 ±1.89 | 0.12 |
30 | 86.91 ± 20.32 | 24.30 ±4.64 | 0.28 | |
10 | 3.55 ± 1.76 | 6.35 ± 0.7 | 1.79 | |
Cellobiose | 20 | 3.89 ± 0.98 | 10.46 ± 0.67 | 2.72 |
30 | 2.76 ± 0.51 | 11.19 ± 0.43 | 4.05 |