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Figure 1 | BMC Biotechnology

Figure 1

From: Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensisin detergents and its ability to degrade the abnormal prion protein

Figure 1

Effect of 0.1% (w/v) surfactants on the activity of Tk-SP. Tk-SP was incubated at 80°C or 90°C for 20 min in 50 mM Tris–HCl (pH 7.5) containing a 0.1% (w/v) surfactant, and the relative activities were determined using azocasein as the substrate. Each experiment was carried out three times, and the average values are shown together with the error bars. Lane 1, EMAL TD; lane 2, EMAL 20C; lane 3, SDS; lane 4, EMULGEN 147; lane 5, EMULGEN LS-114; lane 6, EMULGEN PP-290; lane 7, RHEODOL Tw-0120 V; lane 8, SANISOL C; lane 9, AMPHITOL 24B; lane 10, AMPHITOL 20Y-B; lane 11, AMPHITOL 20 N.

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BMC Biotechnology

ISSN: 1472-6750

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