Table 5 Summary of the optimized process used in this study for tyrosinase production
From: High level production of tyrosinase in recombinant Escherichia coli
Parameters | Tyrosinase production |
|---|---|
Strain | JM109 (pMFvpt) |
Medium | Minimal medium containing glycerol and NZ-amine |
Temperature (°C) | 32 |
pH | 6.9 |
Dissolved oxygen (%) | 30 |
Cultivation | Fed-batch culture with exponential feeding |
Feeding medium | Glycerol and NZ-amine |
Feeding rate (h-1) | 0.3 |
Induction stage | At OD600 value of 30-40 |
Harvest | End of the exponential growth |