High level production of tyrosinase in recombinant Escherichia coli

BMC Biotechnology

Table 1 Comparison of different E. coli hosts for tyrosinase production

Hosts ^a	μ_max(h^-1) ^b	OD_600max^c	Max. act. (U mL^-1) ^d
DH5α	0.26 ± 0.04	7.7 ± 0.4	0.44 ± 0.05
DH5α + IPTG	0.27 ± 0.03	8.0 ± 0.3	0.44 ± 0.01
BLR	0.60 ± 0.01	8.9 ± 0.4	0.03 ± 0.00
BLR + IPTG	0.57 ± 0.02	7.5 ± 0.6	0.03 ± 0.00
JM109	0.60 ± 0.02	7.4 ± 0.3	0.03 ± 0.00
JM109 + IPTG	0.59 ± 0.03	7.7 ± 0.4	0.07 ± 0.01

^a. Cells were grown in shake flasks at 37°C with or without 1 mM IPTG induction at the early growth phase (OD₆₀₀ ≈ 0.6).
^b. μ_max: the maximal growth rate.
^c. OD_600max: the maximal optical density measured at 600 nm.
^d. Max. act.: the maximal volumetric activity of tyrosinase in units per mL of culture broth.

ISSN: 1472-6750