Figure 2From: High level production of tyrosinase in recombinant Escherichia coliInfluence of induction stages on tyrosinase production. The freshly transformed E. coli JM109 (pMFvpt) recombinant cells were grown in shake flasks at 37°C and induced with 1 mM IPTG at the early (from the beginning of the cultivation), mid (6 h cultivation) or late (8 h cultivation) growth phase. The extracts of E. coli JM109 (pMFvpt) were prepared and analyzed by SDS-PAGE. Lanes 1–4, samples from induction at the beginning of the cultivation; Lanes 5–8, samples from induction at mid-growth phase; lanes 9–11, samples from induction at late-growth phase. The arrow indicates the position of tyrosinase in the gel. Molecular weight standards are shown on the left.Back to article page