Figure 5From: Molecular cloning of a novel bioH gene from an environmental metagenome encoding a carboxylesterase with exceptional tolerance to organic solventsEffect of temperature and pH on BioHx activity and stability. Relative activity of purified BioHx was determined at different temperatures (A) or pH (C) using pNP-butyrate as the substrate at 405 nm. Remaining enzyme activity was measured at 30°C and pH 8.0 after incubating purified BioHx at different temperatures for 30 min (B), or pH at 4°C for 12 h (D). All determinants were performed in triplicate.Back to article page