Figure 4From: Molecular cloning of a novel bioH gene from an environmental metagenome encoding a carboxylesterase with exceptional tolerance to organic solventsSDS-PAGE analysis of the bioHx gene product. Protein molecular weight marker (Tiangen, Cat. No. DPP530S) (Lane 1), soluble proteins of E. coli BL21 (pET28a) (Lane 2) and E. coli BL21 (pET28a-bioHx) (Lane 3) cultured and induced under the same condition at 21°C for 20 h, and purified recombinant BioHx (29.27 kDa) (Lane 4).Back to article page