Figure 3

Expression patterns. Reporter expression patterns in adult hermaphrodites transgenic for fosmid-based constructs that tag each of the genes F09E5.3 (A-F), F09E5.15 (prdx-2) (G-N) and EEED8.6 (ccpp-6) (O-Q) individually, or collectively (R-U). F-CFP (B, D, R), mTFP1 (E, F), F-YFP (H, I, K, L, M, N, S) and Mc-mCherry (P, Q, T) and merged F-CFP/F-YFP/Mc-mCherry (U) distributions are presented. DIC was used to observe anatomical details either alone (A, C, G, J, O) or after merging with the associated FP image(s) (I, L, M, N, Q). The C. elegans strains photographed were CTD1059 (A-D), CTD1050 (E, F), CTD1051 (G-N), and CTD1052 (O-Q) and CTD1055 (R-U) (Additional file 1: Table S7). Images were captured at 100x (A, B, E, F, J, K, L, R, S, T, U) or 400x (C, D, G, H, I, O, P, Q) magnification and fluorescence image capture times were 2 msec (A, C, G, H, J, K, O, U), 5 msec (S), 250 msec (E, F) or 1 sec (B, D, P, R, T). Confocal images (M, N) were captured at 400x magnification. Mc-mCherry expression in the anterior dendritic projections and cell bodies of one pair of the four head cephalic neurons are indicated (arrowheads) in panel P. The location of these same cell bodies is also indicated in panel T (arrowheads). In panels R-U the approximate outline of the imaged worm is provided and, in addition, in panel T a region of granular intestinal autofluorescence prominent following the 1 sec excitation of Mc-mCherry is also outlined.