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Table 4 Electroporation results PKC2109

From: Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig

Cell number

DNA amount [μg]

Quality

Cells in suspension

Fluor. intensity

Transfection efficiency [%]

0.5x106

1

3

1

+

28

5

3

2

+

46

10

3

2

++

47

1.0x106

1

4

2

+

24

5

4

2

+

22

10

4

2

++

30

20

5

2

+++

54

  1. Scale for the assessment of cell quality: 1- excellent to good; 2- cells are bit stretched/strongly fibroblast-like; 3- stretched cells with vacuoles; 4- very spindle-shaped morphology 5- extreme spindle-shaped cells with vacuoles; Scale for the assessment of cells in suspension: 0- none; 1- low number of cells; 2- a lot of cells; Fluor. = Fluorescence. Endotoxin-free purified DNA was used; Voltage: 230V.
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BMC Biotechnology

ISSN: 1472-6750

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