Table 1 qPCR Primers Used in the Present Study
From: Establishment of automated culture system for murine induced pluripotent stem cells
Gene | Forward Primer | Reverse Primer | (UPL) probe |
|---|---|---|---|
Oct3/4 | GTTGGAGAAGGTGGAACCAA | CTCCTTCTGCAGGGCTTTC | #95 |
Sox2 | TCCAAAAACTAATCACAACAATCG | GAAGTGCAATTGGGATGAAAA | #63 |
Klf4 | CGGGAAGGGAGAAGACACT | GAGTTCCTCACGCCAACG | #62 |
c-Myc | CCTAGTGCTGCATGAGGAGA | TCTTCCTCATCTTCTTGCTCTTC | #77 |
Nanog | TTCTTGCTTACAAGGGTCTGC | AGAGGAAGGGCGAGGAGA | #110 |
Zfp42 | TCTTCTCTCAATAGAGTGAGTGTGC | GCTTTCTTCTGTGTGCAGGA | #71 |
Ttr | CCTGCAGCCGCATTAAGT | GATGGTGTAGTGGCGATGG | #76 |
Afp | CATGCTGCAAAGCTGACAA | CATGCTGCAAAGCTGACAA | #63 |
Myh6 | CGCATCAAGGAGCTCACC | CCTGCAGCCGCATTAAGT | #6 |
Brachyury | CAGCCCACCTACTGGCTCTA | GAGCCTGGGGTGATGGTA | #100 |
Gfap | TCGAGATCGCCACCTACAG | GTCTGTACAGGAATGGTGATGC | #67 |
Nes | TCCCTTAGTCTGGAAGTGGCTA | GGTGTCTGCAAGCGAGAGTT | #67 |
CD13 | AATCTCATCCAGGGAGTGACC | TCCGCTTTAAACTGCTCCAG | #82 |
Gapdh | AGCTTGTCATCAACGGGAAG | TTTGATGTTAGTGGGGTCTCG | #9 |