Table 1 Summary of purification of laccase secreted by P. pastoris pPICZαB lcc -5
Purification Steps | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification fold |
|---|---|---|---|---|---|
Crude extract | 370 | 18 | 20.5 | 100 | 1 |
Ammonium sulfate precipitation | 138.24 | 3.6 | 38.4 | 37.3 | ~1.9 |
Sephadex G75 + SuperdexG200 | 90 | 1.06 | 84.9 | 24.3 | ~4.0 |