Skip to main content

Table 5 Cold-induced cell injury to LLC-PK 1 cells

From: Aggravation of cold-induced injury in Vero-B4 cells by RPMI 1640 medium – Identification of the responsible medium components

 

LDH release (%)

Solution

Without deferoxamine

With deferoxamine

L15

45 ± 20

1 ± 0

MEM

35 ± 17

1 ± 1

M199

32 ± 17

1 ± 1

RPMI

40 ± 09

2 ± 0

RPMI + EtOH

38 ± 09

 

RPMI + tfp + Fructose

09 ± 03

 

KH

49 ± 13

 

KH(Ca-,P+)

56 ± 15

 

KHG

55 ± 13

2 ± 0

KHG(Ca-,P+)

64 ± 14

2 ± 0

KHG(Ca-,P+) + EtOH

63 ± 15

 

KHG(Ca-,P+) + tfp + Fructose

08 ± 05

 
  1. LLC-PK1 cells were incubated at 4°C in RPMI 1640, DMEM, L-15, M199, Krebs-Henseleit buffer (KH), KH + glucose (11.1 mM; KHG) and modified KH buffer containing a low Ca2+ concentration and a high inorganic phosphate concentration without (KH(Ca-,P+)) or with glucose (KHG(Ca--,P+)), all with or without the iron chelator deferoxamine (1 mM) for 48 hours. Part of the incubations were performed in the presence of the inhibitors of mitochondrial permeability transition, trifluoperazine (tfp; 20 μM) plus fructose (10 mM). Ethanol was used as solvent control (+ EtOH). Cell injury was assessed by release of lactate dehydrogenase (LDH; n = 4).
Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us