Figure 3From: Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology Response surface 3D contour plots of the combined effects of (A) Number of passes and Biomass concentration; (B) Number of passes and Pulse pressure and (C) Biomass concentration and Pulse pressure on specific protein release of HBsAg from cell disruption of P. pastoris cells. Back to article page