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Table 3 Mutations tolerated at the MIV motif

From: Structural insights from random mutagenesis of Campylobacter jejunioligosaccharyltransferase PglB

PglB variant

ELISA EPA-CP5

PglB polypeptide

 

Abs. 450 nm

% of wt

anti-HA, % of wt

wt

0.57 (±0.03)

100

100 (±13)

mut

0.20 (±0.01)

0

56 (±7)

I571V

0.51 (±0.05)

85

89 (±28)

I571V V575I

0.52 (±0.04)

87

85 (±11)

I571V V575L

0.46 (±0.04)*

70

84 (±7)

I571F V575L

0.32 (±0.01)*

34

96 (±31)

I571C V575I

0.41 (±0.03)*

58

65 (±13)

  1. ELISA absorbance measured against air. Average values and standard deviations of at least n = 3 replicate mini-well cultures.
  2. * Significant difference to wt and mut, P value of Student’s T-test < 0.001.
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BMC Biotechnology

ISSN: 1472-6750

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