Table 1 Freezing protocols used

1

0,3°C/min to −100°C

Store in vapour phase at −134°C

2

1°C/min to −80°C

Store in vapour phase at −134°C

3

1°C/min to - 30°C

5°C/min to −80°C

xStore in vapour phase at −134°C

4

1°C/min to −20°C

5°C/min to −40°C

10°C/min to −80°C

20°C/min to −100°C

Store in vapour phase at −134°C

5

1°C/min to −6°C

25°C/min to −50°C

10°C/min to −90°C

Store in vapour phase at −134°C

6

Directly into the vapour phase

7

99°C/min to −100°C

Store in vapour phase at −134°C