Field performance of transgenic citrus trees: Assessment of the long-term expression of uidA and nptIItransgenes and its impact on relevant agronomic and phenotypic characteristics

Table 3 The effects of the ‘line’, ‘transgene’ and ‘ploidy’ factors on the fruit quality in the citrange lines

	Fruit quality parameter (dependent variable)
Source	Weight				Volume				Caliber				Color Index				Juice Content¹				MI (TSS/TA)
	S1	S2	S3	S4	S1	S2	S3	S4	S1	S2	S3	S4	S1	S2	S3	S4	S1	S2	S3	S4	S1	S2	S3	S4
Factor /ANOVA ²
Line	***	*	**	NS	***	*	***	NS	***	*	***	NS	NS	NS	NS	–	**	***	*	-	*	*	NS	-
Plant(Line)	***	***	***	***	***	***	***	***	***	***	***	***	***	***	***	–	*	NS	***	-	***	***	*	-
Contrasts to test ‘transgene’ effect ³
C1 vs CC	***	NS	NS	NS	***	NS	NS	NS	***	NS	NS	NS	NS	NS	NS	–	*	NS	NS	–	NS	NS	NS	–
C3 vs CC	*	NS	NS	NS	NS	NS	NS	NS	*	NS	NS	NS	NS	NS	NS	–	*	NS	NS	–	NS	NS	NS	–
C4 vs CC	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	–	NS	NS	NS	–	NS	*	NS	–
C5 vs CC	**	NS	NS	NS	**	NS	NS	NS	**	NS	NS	NS	NS	NS	NS	–	NS	NS	*	–	NS	NS	NS	–
C6 vs CC	**	NS	NS	NS	*	NS	NS	NS	*	NS	NS	NS	NS	NS	NS	–	*	NS	NS	–	NS	NS	NS	–
C8 vs CC	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	NS	–	NS	NS	NS	–	NS	NS	NS	–
Contrasts to test ‘ploidy’ effect ⁴
T-2n vs T-4n	***	***	***	*	***	***	***	**	***	***	***	**	NS	NS	NS	–	*	***	**	-	*	**	*	-

¹The “juice content” variable was transformed prior to the analyses to fit the data to a normal distribution.
²ANOVA to test the effects of Line on each fruit quality variable. Independent statistical analyses were performed for each fruit quality parameter and season.
³Contrasts to test for significant differences between each transgenic diploid line and their respective control line (CC) using Dunnett’s test. In the absence of a tetraploid control line, transgenic lines C2 and C7 (tetraploids) were excluded from this analysis to avoid confounding effects.
⁴Planned comparisons to test for significant differences between the average of all transgenic diploid lines (T-2n) and the average of all transgenic tetraploid lines (T-4n). Because only one control line (CC) was available for the Carrizo citrange plants, which was diploid, the data from this line were excluded from this analysis to avoid confounding effects.
MI, maturity index; TSS, total soluble solids; TA, titratable acidity; S1, season 2004; S2, season 2005; S3, season 2006; S4, season 2007.
-, Not measured; *p < 0.01; **p < 0.001; ***p < 0.0001; NS, not significant.

ISSN: 1472-6750