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Table 1 Activity screening of putative CAZy-related genes

From: Glycoside Hydrolases from a targeted Compost Metagenome, activity-screening and functional characterization

ORF

Family

Predicted activity

Active in screen

JMC02101a

GH5

cellulase

No

JMC03259

GH10

endoxylanase

No

JMC14824

GH10

endoxylanase

No

JMC04931

GH10

endoxylanase

No

JMC07159

GH10

endoxylanase

No

JMC07447

GH10

endoxylanase

No

JMC37744

GH10

endoxylanase

Yes

JMC01245

GH11

endoxylanase

Yes

JMC09349

GH29

α-fucosidase

Yes

JMC02101b

GH30

β-glucosidase

No

JMC44805

GH39

β-xylosidase

No

JMC25406

GH43

β-xylosidase/α-arabinofuranosidase

Yes

JMC04168

GH43

β-xylosidase/α-arabinofuranosidase

Yes

JMC35591

GH43

β-xylosidase

No

JMC02364

GH51

α-arabinofuranosidase

No

JMC00766

GH51

α-arabinofuranosidase

No

JMC15193

GH51

α-arabinofuranosidase

Yes

JMC04920

GH62

α-arabinofuranosidase

No

JMC10050

GH67

α-glucuronidase

No

JMC04701

CE10

pectin acetylesterase

No

JMC16911

CE12

acetyl xylan esterase

Yes

JMC10774

CE12

acetyl xylan esterase

No

  1. Twenty-two ORFs from 12 CAZy families were screened for expression and activity. All of the ORFs could be expressed in soluble form in E. coli and seven of twenty-two ORFs demonstrated the predicted activity.