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Figure 2 | BMC Biotechnology

Figure 2

From: Glycoside Hydrolases from a targeted Compost Metagenome, activity-screening and functional characterization

Figure 2

All of the codon-optimized genes are highly expressed in E. coli. Twenty-two genes in each of three expression vectors were expressed in E. coli. pET57 expression lysates (panel A), pET60 expression lysates (panel B), and pVP16 expression lysates (panel C) were analyzed with a LabChip GXII Protein Assay (Caliper Life Sciences, Hopkinton, MA). Ordering of samples for each panel is alphanumeric for each vector backbone. Expression of all genes was confirmed in each of the three vectors.

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BMC Biotechnology

ISSN: 1472-6750

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