Figure 1
From: Solution-based targeted genomic enrichment for precious DNA samples
Reduced requirement for input DNA using this method. A single gDNA sample was taken through the protocol using either 3,000 ng (curve A), 1,500 ng (curve B), 750 ng (curve C), 500 ng (curve D), and 375 ng (curve E) starting material and 6 cycles of PCR amplification. A) Bioanalyzer trace after shearing step, B) Bioanalyzer trace after pre-hybridization amplification showing no detriment to overall library preparation yield for starting material greater than 500 ng.